Origin: human, glioblastoma.
Human Heredity 1971. 21: 238.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,
split ratio 1:3, optimal population density 1.0õ105 cells/cm2
cryoconservation -DMEM 70%, FBS 20%, glycerol 10%, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 50% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 51-68 chromosomes, modal number o
f chromosomes 64-66, Y-chromosome is presented, without markers (routine, differential dye,
C-banding), number of polyploid cells 6.0%.
isoenzymes G6PD, B; PGM1, 1.
Applications: virology, tumorigenicity.