GL-6 (251-MG)

Origin: human, glioblastoma.

Human Heredity 1971. 21: 238.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:3, optimal population density 1.0105 cells/cm2

cryoconservation -DMEM 70%, FBS 20%, glycerol 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 50% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 51-68 chromosomes, modal number o

f chromosomes 64-66, Y-chromosome is presented, without markers (routine, differential dye,

C-banding), number of polyploid cells 6.0%.

Tumorigenicity: tumorigenic

Other properties:

isoenzymes G6PD, B; PGM1, 1.

Applications: virology, tumorigenicity.

Collections: MWIIW.