FLECh 997/11

Origin: human, lung of normal human embryo (designed in SPBII)

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - BS 10 %

subculture procedure - cells detachment using 50 mg chymopsine in 500 ml EDTA 0.04 %, split ratio 1 : 2

cryoconservation - growth medium, 30 % BS, 5 % DMSO, 1.5 - 2.0x106 cells/ml in ampule.

Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis.

Other properties:

virus susceptibility: parainfluenza types 2 and 3, RS, adenovirus, herpes simplex, ECHO, Coxsackie,

vesicular stomatitis, cytomegalovirus

Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset of the

decline in proliferation.

Applications: cell biology, virology, interferone titration

Collections: SPBII.

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