Origin: human, lung of normal human embryo (designed in SPBII, NPKK 005)
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - BS 10 %
subculture procedure - cells detachment using 50 mg chymopsine in 500 ml EDTA 0.04 %, split ratio 1 : 2
cryoconservation - growth medium, 30 % BS, 5 % DMSO, 1.5 - 2.0x106 cells/ml in ampule.
Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
virus susceptibility: parainfluenza types 2 and 3, RS, adenovirus, herpes simplex, ECHO, Coxsackie, v
esicular stomatitis, cytomegalovirus.
Finite lifetime culture, cells are capable of attaining 49-50 population doubling before onset of the
decline in proliferation.
Applications: cell biology, virology, interferone titration.