Origin: human, bladder carcinoma.
Nature 1976. 264: 60; Tissue Antigens 1978. 11: 279.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0x105 cells/cm2
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 81-92 chromosomes,
modal number of chromosomes 88 without markers (routine and differential dye,
C-banding), there are some microchromosomes.
G6PD, B; PGM 3, 1.
HLA cell line phenotype A (2, w32); B (5, 12), C (w2, w3), Ek-2; Ek-10.
Applications: virology, biochemistry, cell biology, oncology.