EJ (MGH-U1)

Origin: human, bladder carcinoma.

Nature 1976. 264: 60; Tissue Antigens 1978. 11: 279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0x105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 81-92 chromosomes,

modal number of chromosomes 88 without markers (routine and differential dye,

C-banding), there are some microchromosomes.

Tumorigenicity: tumorigenic

Other properties:

G6PD, B; PGM 3, 1.

HLA cell line phenotype A (2, w32); B (5, 12), C (w2, w3), Ek-2; Ek-10.

Applications: virology, biochemistry, cell biology, oncology.

Collections: MWIIW.

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