Origin: human, normal conjunctiva.
Proc.Soc.Exp.Biol.Med. 1954. 87: 440; J.Exp.Med. 1961. 113: 95; Virology 1965. 26: 478;
Nature 1976. 259: 211; In Vitro 1978. 14: 469.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,
split ratio 1:4-1:5, optimal population density 1.0x105 cells/ml
cryoconservation - BME 70%, BS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation: 98 % (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46 , variability in the range between 62-72 chromosomes, modal number of chromosomes 71,
number of markers-4, specific for Hela (NN1,2,3,4, differential dye), the cells have microchromosomes,
number of polyploid cells 16.0%.
Plating efficiency: 17% (ATCC)
virus susceptibility: influenza A,B; poliovirus 1; vesicular stomatitis.
This line probably was contaminated with Hela cells.
Collections: ATCC CCL 20.2; ECACC 88021103; MWIIW