Clone 1-5c-4

Origin: human, normal conjunctiva.

Proc.Soc.Exp.Biol.Med. 1954. 87: 440; J.Exp.Med. 1961. 113: 95; Virology 1965. 26: 478;

 Nature 1976. 259: 211; In Vitro 1978. 14: 469.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin,

split ratio 1:4-1:5, optimal population density 1.0x105 cells/ml

cryoconservation - BME 70%, BS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 98 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 , variability in the range between 62-72 chromosomes, modal number of chromosomes 71,

number of markers-4, specific for Hela (NN1,2,3,4, differential dye), the cells have microchromosomes,

number of polyploid cells 16.0%.

Plating efficiency: 17% (ATCC)

Other properties:

virus susceptibility: influenza A,B; poliovirus 1; vesicular stomatitis.

Isoenzymes G6PD,A

This line probably was contaminated with Hela cells.

Applications: virology

Collections: ATCC CCL 20.2; ECACC 88021103; MWIIW

 

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