Origin: human, normal liver.
Proc.Soc.Exp.Biol.Med. 1954. 87: 440; Natl. Cancer Inst. Monograph. 1962. 7: 249;
In Vitro 1975. 10: 374; J.Cell Physiol. 1977. 91: 119; Science 1978. 199: 567; Tissue Antigens 1978. 11: 278.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin, split ratio 1:3-1:5,
optimal population density 0.5-1.0õ105 cells/ml
cryoconservation -BME 70%, FBS 20%, glycerol 10%, 2.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 96% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 65-74 chromosomes, modal number of chromosomes 71,
number of markers - 1, large submetacentric chromosome (routine dye); 4 - specific for Hela (NN1,2,3,4, differential dye).
Plating efficiency: 20% (ATCC)
virus susceptibility: polioviruses, adenoviruses, vesicular stomatitis, influenza.
Synthesis of human liver proteins especially liver alkaline phosphatase.
This line probably was contaminated with Hela cells.
Applications: virology, tumorigenicity.
Collections: ATCC CCL 13; ECACC 88021102; MWIIW.