Chang liver

Origin: human, normal liver.

Proc.Soc.Exp.Biol.Med. 1954. 87: 440; Natl. Cancer Inst. Monograph. 1962. 7: 249;

In Vitro 1975. 10: 374; J.Cell Physiol. 1977. 91: 119; Science 1978. 199: 567; Tissue Antigens 1978. 11: 278.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02% with 0.1 mg/ml chimopsin, split ratio 1:3-1:5,

optimal population density 0.5-1.0105 cells/ml

cryoconservation -BME 70%, FBS 20%, glycerol 10%, 2.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 96% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 65-74 chromosomes, modal number of chromosomes 71,

number of markers - 1, large submetacentric chromosome (routine dye); 4 - specific for Hela (NN1,2,3,4, differential dye).

Plating efficiency: 20% (ATCC)

Other properties:

virus susceptibility: polioviruses, adenoviruses, vesicular stomatitis, influenza.

Synthesis of human liver proteins especially liver alkaline phosphatase.

This line probably was contaminated with Hela cells.

Applications: virology, tumorigenicity.

Collections: ATCC CCL 13; ECACC 88021102; MWIIW.

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