Origin: human, colon, carcinoma.

Cancer Res. 1979. 39: 4914.

Morphology: round cells

Mode of cultivation: semisuspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - split ratio 1:3, optimal population density 3.0-9.0105 cells/cm2

cryoconservation - growth medium, 10% DMSO, 9.0106 cells/ml in ampule

Viability after cryoconservation: 85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46 , variability in the range between 49-61 chromosomes, modal number of chromosomes 52,

markers - 9-12 (differential dye), double minute chromosomes (ATCC).

Plating efficiency: 12% ( ATCC)

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes PGM1,1; PGM3,1; G6PD, B; PEP-D,1; PGD, A; ES D, 1

Serotonin, epinephrine, AKTG, NPP, PTH production

Applications: biochemistry, biophysics, endocrinology.

Collections: ATCC CCL 220.1; ECACC 87101501; SPBIC.