CFTE 29o-

Origin: human, tracheal epithelium, cells were transfected with pSVori- plasmid.

Am.J.Respir.Cell Mol.Biol. 1993. 8; 522-529.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:2 - 1:4

cryoconservation - FBS 50%, EMEM 40%, DMSO 10%, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Plating efficiency: 30%

Other properties:

keratin expression.

Homozygous D F508-mutation (cystic fibrosis - recessive genetical disease)

Applications: genetical transformation and hereditary diseases studies, cell biology.

Collections: SPBIC.