Origin: human, tracheal epithelium, cells were transfected with pSVori- plasmid.
Am.J.Respir.Cell Mol.Biol. 1993. 8; 522-529.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS 10%
other components - NEAA 1% (EMEM)
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),
split ratio 1:2 - 1:4
cryoconservation - FBS 50%, EMEM 40%, DMSO 10%, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Plating efficiency: 30%
Homozygous D F508-mutation (cystic fibrosis - recessive genetical disease)
Applications: genetical transformation and hereditary diseases studies, cell biology.