Origin: human, acute B-lymphoblastic leukemia, peripheral blood
Cancer Res. 1967. 27: 2479-24-82
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 5.0õ105 cells/cm2
cryoconservation - growth medium 5-10% DMSO, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 75% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=46 , variability in the range between 42-47, modal number of chromosomes 46,
diploid, normal human karyotype (46, XY). Number of poliploid cells 1% (ATCC).
Ig non synthesised.
Isoenzymes - G6PD, B.
Erythrocyte rosette tests: E, 0; EA, 6%; EAC, 23%.
HLA cell line phenotype A1, A2, B12, B17, Cw2.
Positive for EBNA
Applications: immunology, cell biology.
Collections: ATCC CCL 120; ECACC 89090405; SPBIC.