CCRF-SB

Origin: human, acute B-lymphoblastic leukemia, peripheral blood

Cancer Res. 1967. 27: 2479-24-82

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 5.0105 cells/cm2

cryoconservation - growth medium 5-10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46 , variability in the range between 42-47, modal number of chromosomes 46,

diploid, normal human karyotype (46, XY). Number of poliploid cells 1% (ATCC).

Other properties:

Ig non synthesised.

Isoenzymes - G6PD, B.

Erythrocyte rosette tests: E, 0; EA, 6%; EAC, 23%.

HLA cell line phenotype A1, A2, B12, B17, Cw2.

Positive for EBNA

Applications: immunology, cell biology.

Collections: ATCC CCL 120; ECACC 89090405; SPBIC.

 

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