AMN (AMH)

Origin: human, normal amnion.

Submitted by Orlova T.G. Trudy MRIVP (Russ.), M., 1961. 2: 330.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,

optimal population density 0.6-0.8x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 51-60 chromosomes, modal number of chromosomes 55-58.

Plating efficiency: 60 %

Other properties:

virus susceptibility: polioviruses 1, 2, 3; Coxsackie A and B; ECHO; human adenoviruses 2, 4, 7.

Applications: virology.

Collections: ESCC

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