Origin: human, normal amnion.
Submitted by Orlova T.G. Trudy MRIVP (Russ.), M., 1961. 2: 330.
Mode of cultivation: monolayer
Conditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:4-1:6,
optimal population density 0.6-0.8x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.0-2.0õ106 cells/ml in ampule
Viability after cryoconservation: 80-90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 51-60 chromosomes, modal number of chromosomes 55-58.
Plating efficiency: 60 %
virus susceptibility: polioviruses 1, 2, 3; Coxsackie A and B; ECHO; human adenoviruses 2, 4, 7.