Int.J.Cancer 1967. 2: 434; Human Heredity 1971. 21: 238; Nature 1976. 264: 60; Tissue Antigens 1978. 11: 279.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0õ105 cells/ml
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation: 97% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH and G6PD) analysis
Karyology: 2n= 46, variability in the range between 70-80 chromosomes, modal number
of chromosomes 74, number of markers - 1, submetacentric with the second
constriction at the long arm (routine dye), the cells have microchromosomes.
isoenzymes G6PD, B; PGM1, 2.
HLA cell line phenotype À (1, 2); Â (12, w40); C (w2).
Not contaminated with Hela cells.
Applications: virology, tumorigenicity.