2T

Origin: human, osteosarcoma.

Int.J.Cancer 1967. 2: 434; Human Heredity 1971. 21: 238; Nature 1976. 264: 60; Tissue Antigens 1978. 11: 279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0105 cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 97% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology: 2n= 46, variability in the range between 70-80 chromosomes, modal number

of chromosomes 74, number of markers - 1, submetacentric with the second

constriction at the long arm (routine dye), the cells have microchromosomes.

Tumorigenicity: tumorigenic

Other properties:

isoenzymes G6PD, B; PGM1, 2.

HLA cell line phenotype (1, 2); (12, w40); C (w2).

Not contaminated with Hela cells.

Applications: virology, tumorigenicity.

Collections: MWIIW.

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