HUMAN CELL LINES
Origin: human, embryonal kidney , cell transformed with human adenovirus
type 5 (Ad 5) DNA.
Gen. Virology 1977. 36:59; Virology 1977. 77: 319.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS (or heat inactivated HS) 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:2 - 1:3),
split ratio 1:2 - 1:3, optimal population density 3.0-5.0x104 cells/cm2,
cell detach at room temperature and may take several days to reattach.
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90-95% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n=46, modal number of chromosomes 64,
number of markers - 7 (differential dye), number of polyploid cells 4.2% (ATCC).
virus susceptibility: human adenovirus type 5, astrovirus.
Contain and express the transforming genes of Ad5.
Applications: biotechnology (human adenovirus titration), virology, transformation
Collections: ATCC CRL 1573; ECACC 85120602; MWIIW; SPBIC.