HUMAN CELL LINES

293

Origin: human, embryonal kidney , cell transformed with human adenovirus

 type 5 (Ad 5) DNA.

Gen. Virology 1977. 36:59; Virology 1977. 77: 319.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS (or heat inactivated HS) 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:2 - 1:3),

 split ratio 1:2 - 1:3, optimal population density 3.0-5.0x104 cells/cm2,

cell detach at room temperature and may take several days to reattach.

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

 

Viability after cryoconservation: 90-95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46, modal number of chromosomes 64,

number of markers - 7 (differential dye), number of polyploid cells 4.2% (ATCC).

Other properties:

virus susceptibility: human adenovirus type 5, astrovirus.

Contain and express the transforming genes of Ad5.

Applications: biotechnology (human adenovirus titration), virology, transformation

Collections: ATCC CRL 1573; ECACC 85120602; MWIIW; SPBIC.

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