mouse BALB/c, fibrosarcoma induced by methylcholathrene.
Proc.Soc.Exp.Biol.Med. 1973. 144: 813; J.Natl.Cancer Inst. 1984. 72: 23-29;
Blood 1985. 65: 8-14.
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640 or DMEM
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3-1:6,
optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
70% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
the line is highly sensitive, after pretreatment with actinomycin D, to human cytotoxic
monocytes, to human TNF and to
cytotoxicity, tumorigenicity, cell biology.
Collections: ATCC CRL 1751; ECACC 87022501; DSM (ACC 25); ICLC ATL 96004; SPBIC.