Wehi 164

Origin: mouse BALB/c, fibrosarcoma induced by methylcholathrene.

            Proc.Soc.Exp.Biol.Med. 1973. 144: 813; J.Natl.Cancer Inst. 1984. 72: 23-29;

Blood 1985. 65: 8-14.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - RPMI 1640 or DMEM

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3-1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Other properties:

the line is highly sensitive, after pretreatment with actinomycin D, to human cytotoxic

monocytes, to human TNF and to lymphotoxin.

Applications: cytotoxicity, tumorigenicity, cell biology.

Collections: ATCC CRL 1751; ECACC 87022501; DSM (ACC 25); ICLC ATL 96004; SPBIC.