mouse A/Sn, lymphoma induced in vivo by MLV.
Eur. J. Immunol. 1975. 5: 112-117.
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640
other components - HEPES 0.01M (MWIIW)
optimal population density
cryoconservation - growth medium (may add 40% FBS),
4.0-6.0õ106 cells/ml in ampule
Viability after cryoconservation:
80-90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 40-47 chromosomes,
modal number of chromosomes 43 without markers (routine and differential dye,
number of polyploid cells 2.5%.
this cell line is sensitive to the cytotoxic activity of NK cells.
The cells not discovered of markers B- and T-lymphocytes (MWIIW).
NK assay, cytotoxicity.
ATCC TIB 160; ECACC 86022801; DSM ACC 96; MWIIW; SPBIC.