Origin: mouse A/Sn, lymphoma induced in vivo by MLV.

Eur. J. Immunol. 1975. 5: 112-117.

Morphology:  lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -    FBS 10%

other components -  HEPES 0.01M (MWIIW)

subculture procedure   optimal population density 3.0-9.0105 cells/ml

cryoconservation - growth medium (may add 40% FBS),

10% DMSO, 4.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 40-47 chromosomes,

 modal number of chromosomes 43 without markers (routine and differential dye,

C-banding), number of polyploid cells 2.5%.

Other properties:

this cell line is sensitive to the cytotoxic activity of NK cells.

The cells not discovered of markers B- and T-lymphocytes (MWIIW).

Applications:  NK assay, cytotoxicity.

Collections: ATCC TIB 160; ECACC 86022801; DSM ACC 96; MWIIW; SPBIC.