Origin: rat  Wistar, sarcoma, subline of cell line XC derived from sarcoma,

induces in vivo by Raus sarcoma .

            Submitted  from Cardiological Scientific Centre. Moscow. 1979.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:10

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and immunofluorescent analysis

Karyology:   2n=42 , variability in the range between 40-45 chromosomes,

modal number of chromosomes  42-43, number of markers -10 (differential dye),

 number of polyploid cells 70%    

Plating efficiency:  68 %

Applications: cell biology

Collections:  SPBIC.