XC

Origin: rat, sarcoma.

            Nature 1960. 168: 980; Folia Biol. 1961. 7: 46; 1962. 8: 221; 1963. 9: 77;

Neoplasma 1962. 9: 104; PNAS 1969. 63: 753; Virology 1970. 42: 1136.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -    BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:4-1:6, optimal population density   1.0105 cells/cm2

cryoconservation - BME 80%, BS 10%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:    84% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 32-52 chromosomes,

modal number of chromosomes 39, number of markers - about 10 (differential dye),

number of polyploid cells 16.0%.

Plating efficiency: 15% (ATCC)

Tumorigenicity:  tumorigenic

Other properties:

virus susceptibility: vesicular stomatitis, MLV.

Contain the RS genome but does not appear to release infectious virus.

Applications: virology, the cells are employed as indicator for detecting the growth o

f murine leukemia viruses in cell cultures.

Collections: ATCC CCL 165; ECACC 88120601; ICLC ATL 96013; MWIIW.