Wehi-3

Origin: mouse BALB/c, myelomonocytic leukemia.

            J.Exp.Med. 1976. 143: 1528-1533; Cancer Res. 1977. 37: 546-550;

J.Immunol. 1977. 119: 950-954; J.Exp.Med. 1981. 154: 1419-1431.

Morphology: macrophage-like

Mode of cultivation: semisuspension

Conditions for cultivation: medium - Iscoveĺs MDM

serum -    FBS 10%

other components -  2-mercaptoethanol 10-5

subculture procedure - optimal population density  1.0-5.0§105 cells/ml

cryoconservation - growth medium,  8%DMSO, 3.0-4.0§106 cells/ml in ampule

Viability after cryoconservation:     70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 72-83 chromosomes,

modal number of chromosomes 75-78, number of markers - 4 metacentric

chromosomes (routine dye), number of polyploid cells 0.8%.

Other properties:

lysozyme, IL-3 and granulocyte CSA production.

Ig and complement receptors.

Applications: immunology, cell biology, chemotherapeutic agents studies.

Collections: ATCC TIB 68; SPBIC.