African green monkey, kidney, clone of Vero 76.
In: « Vero cells - origin, properties and biomedical applications».Depart.
Microbiol. School of Medicine Chiba University, Japan. 1988:
epithelial- and fibroblast-like
Mode of cultivation:
Conditions for cultivation:
medium - BME
FBS 8 -10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3 - 1:5, optimal population density 1.0x105cells/ml
cryoconservation - BME 70%, FBS 20% glycerol 10%,
3.0-5.0ő106 cells/ml in ampule
Viability after cryoconservation:
90 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDG, G6PD) analysis
Karyology: 2n=60, variability in the range between 52-58 chromosomes, modal
number of chromosomes 56, number of markers - 3 (routine and differential dye,
C banding), number of polyploid cells 6.0%.
virus susceptibility: hemorrhagic fever Machupo (Bolivian), Junin (Argentinian),
Lassa (African); karelian fever (Russian);
Collections: ATCC CRL 1586, ECACC 85020206, MWIIW.