Vero C1008

Origin: African green monkey, kidney, clone of Vero 76.

In: « Vero cells - origin, properties and biomedical applications».Depart.

Microbiol. School of Medicine Chiba University, Japan. 1988: 26.

Morphology: epithelial- and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -    FBS  8 -10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3 - 1:5, optimal population density  1.0x105cells/ml

cryoconservation - BME 70%, FBS 20% glycerol 10%, 3.0-5.0ő106 cells/ml in ampule

Viability after cryoconservation:   90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDG, G6PD) analysis

Karyology:   2n=60, variability in the range between 52-58  chromosomes, modal

number of chromosomes 56, number of markers - 3 (routine and differential dye,

C banding), number of polyploid cells 6.0%.

Other properties:

virus susceptibility: hemorrhagic fever Machupo (Bolivian), Junin (Argentinian),

Lassa (African); karelian fever (Russian); Marburg; Ebola.

Applications: virology, biotechnology.

Collections: ATCC CRL 1586, ECACC 85020206, MWIIW.