Vero 76

Origin: African green monkey, kidney, subline of Vero.

            Vero cells - Origin, properties and biomedical applications. Tokyo: Soft Science Publications. 1988. 26-29.

Morphology:   fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  DMEM or EMEM

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA  0.02% (1:3), split ratio 1:8, optimal population density 1.0-3.0x104   cells/cm2

cryoconservation - growth medium, 10 %DMSO, 2.0106 cells/ml in ampule

Viability after cryoconservation: 90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=60, variability in the range between 53-60 chromosomes, modal number of chromosomes 56-57, number of markers - 1 (routine dye), number of polyploid cells  9%.

Other properties:

virus susceptibility: haemorrhagic fever viruses, Ebola.

Applications:  virology, cell biology.

Collections: ATCC CRL 1587; ECACC 85020205; SPBIC.