African green monkey, kidney, subline of Vero.
Vero cells - Origin, properties and biomedical applications. Tokyo: Soft Science Publications. 1988. 26-29.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM or EMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:8, optimal population density 1.0-3.0x104 cells/cm2
cryoconservation - growth medium, 10 %DMSO, 2.0õ106 cells/ml in ampule
Viability after cryoconservation:
90 % (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
2n=60, variability in the range between 53-60 chromosomes, modal number of chromosomes 56-57, number of markers - 1 (routine dye), number of polyploid cells
virus susceptibility: haemorrhagic fever viruses, Ebola.
virology, cell biology.