Vero

Origin: African green monkey, kidney.

            Nippon Rincho 1963. 21: 1209; Arch. GVS Virusforsch. 1969. 27: 379.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  199, EMEM, DMEM or BME

serum -  BS or FBS 5-10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:2), split ratio 1:3-1:10, optimal population density  1.0-3.0x104 cells/cm2

cryoconservation - growth medium (may add 30% FBS or BS),

10 %DMSO or glycerol  1.0-2.0๕106 cells/ml in ampule

Viability after cryoconservation:   77 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological( LDH, G6PD, nucleoside phosphorylase)analysis

Karyology:   2n=60, variability in the range between 53-60 chromosomes,

modal number of chromosomes 57-58, number of markers -3 (routine and differential dye, C banding),

number of polyploid cells  2%    

Plating efficiency:  24 % (SPBIC); 65% (ESCC)

Other properties:

virus susceptibility: ortomixoviruses (influenza); Getah, Ndumu, Pixuna, Ross River, Semliki,

Paramaribo, Kokobera,Modoc, Murutucu, Germiston, Guaroa, Pongola, Tacaribe Arboviruses;

bovine leucosis; bluetongue; adenovirus 12; paramixoviruses ( parainfluenza 1 and 4, measles,

respir.syncytial virus) ; poliovirus 3; rubella; African swine fever virus; reoviruses; herpes

simplex; vesicular stomatitis;  echoviruses; SV 40; SV 5.

Isoenzymes: LDG, G6PD, A, typical  for primate cells.

Applications:  virology, cell biology.

Collections: ภาัั ััL81; ECACC 84113001, 88020401; ICLC ATL 95005;

MWIIW; SPBII; ESCC; SPBIC, MWIEV.