Vero (V)

Origin: african green monkey, kidney, subline of Vero.

Cytology (Russ.) 1996. 2: 241; Vopr.Virusol. (Russ.)  1996. 4: 183.

Morphology: epithelial- and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -    FBS 8%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:4-1:5, optimal population density 1.0105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 2.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 60, variability in the range between 49-58 chromosomes,

modal number of chromosomes 54-55, number of markers - 1 large metacentric

chromosome (routine dye) and 3 markers (differential dye, C-banding), number of polyploid cells 3.0%.

Tumorigenicity:  non tumorigenic

Other properties:

virus susceptibility: herpes simplex 1 and 2, cytomegalovirus, hepariris A, vaccinia.

The keeping of stability of biological properties in the range 178-200 subcultures.

Applications: virology, biotechnology.

Collections: MWIIW