V-79

Origin: Chinese  hamster, lung

            J.Cell Biol.1967.34:684;  Mol. Cell  Biol. 1987. 7 :4218

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  DMEM

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5-1:10, optimal population density  2.0-4.0x104  cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:   88 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 22, variability in the range between 17-23  chromosomes,

 modal number of chromosomes 21, number of markers 11 (differential dye),

 number of polyploid cells  6%

Plating efficiency:  58 % (SPBIC)

Other properties:

The cells have very short  G1 phase of mitotic cycle

Applications:  cell biology, proliferation mechanisms, somatic cell genetics, transformation.

Collections: ECACC 86041102,  SPBIC.