TR

Origin: cattle, trachea.

Folia Biol. 1975. 21:117; Bull. of VIEV, 1983, issue 49: 3-8.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -    FBS 7-10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation - BME 45%, FBS 45%, DMSO 10%,  

3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     76% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 60, modal number of chromosomes 61-63

Other properties:

virus susceptibility: parainfluenza 3; IBR; parvovirus of cattle.

Applications: virology, biotechnology, genetical engineering.

Collections: MWIEV