TR-GFRT-

Origin: cattle, trachea, subline of TR.

            Agricultural Biology (Russ.) 1985. 1: 22-28; Agricultural Biology (Russ.) 1994. 4: 26-33.

USSR Patent N 12914010, 1986.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3-1:4

cryoconservation - culture medium 50%, FBS 40%, DMSO 10%,

3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 60, modal number of chromosomes 61

Other properties:

virus susceptibility: rota-, corona-, herpes viruses, IBR, bovine parainfluenza 3.

Deficient in hypoxanthine guanine phosphoribosyl transferase.

Presence of oncornavirus C.

Applications: virology, biotechnology.

Collections: MWIEV