TK- LM (clone 1D)

Origin:  mouse  C3H/An, connective, subline of LM.

Proc. Natl. Acad. Sci.,  1979. 76: 3755.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -    FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:6, optimal population density  1.0x105 cells/ml

cryoconservation - BME 70%,  FBS 20%, glycerol 10%, 1.0106 cells/ml in ampule

Viability after cryoconservation:   88 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDG, G6PD) analysis

Karyology:   2n=40-, variability in the range between 46-56 chromosomes,

modal number of chromosomes 53, number of markers 5 (routine and (differential dye, C banding).

Other properties:

virus susceptibility: herpes viruses.

Deficient in thymidine kinase.

Applications: virology

Collections: MWIIW