TEK

Origin: bovine, embryo, thymus.

            Bulletin WIEV 1972. 14: 64; 1972. 14: 78.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -    BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 0.5-1.0105 cells/cm2

cryoconservation - BME 80%, BS 10%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 60, variability in the range between 56-64 chromosomes,

modal number of chromosomes 60, pseudodiploid, number of markers - 2-4

metacentric and submetacentric chromosomes (routine dye), number of polyploid cells 2.0%.

Other properties:

virus susceptibility: foot and mouth disease; vesicular stomatitis; Aujesky;

bovine rhynotracheitis; rabies; smallpoxvaccine; horse rhinopneumonia.

Applications: virology.

Collections: MWIIW.