Taurus-1

Origin: calf kidney.

Authorís patent certificate N 1347448 of 22.05.87 deposition number RKKDP/002/SPBII

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMEM

serum -  BS 10 %

subculture procedure - cells detachment using 50 mg chymopsine in 500 ml EDTA 0.04 %,

split ratio 1 : 3

cryoconservation - 85 % growth medium, 10 % BS, 5 % DMSO,

1.0 - 2.0x106 cells/ml in ampule.

Viability after cryoconservation: 70 - 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:   karyological and isoenzymatic analysis (LDH, G6PD)

Karyology:  aneuploid cells (24 %), polyploid cells (2 %), modal number of

chromosomes 57, number of markers - 3.

Other properties:

virus susceptibility:  inzluenza, parainfluenza, adenoviruses, cattle viruses (

infectious rhynotracheitis, adenovirus, diarrhea)

Applications: cell biology, virology, biotechnology (test-systems preparation, accumulation of viral mass)

Collections: SPBII; ESCC.