Origin: sheep, heart.

USSR Patent N1747077, 1992.

Morphology: epithelial-like and fibroblast-like.

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -    FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation - DMEM 50%, FBS 40%, DMSO 10%,

 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     73% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 54, diploid.

Other properties:

virus susceptibility: visna-maedi; RSV.

Applications: virology, biotechnology, cell biology.

Collections: MWIEV