SPEV-G

Origin: pig, kidney, subline of SPEV.

Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.5% MWPH

serum -    BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:4-1:5

cryoconservation - culture medium 50%, BS 40%, DMSO 10%,  

2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     78% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 38, modal number of chromosomes 40.

Other properties:

virus susceptibility: rota- and coronaviruses of swine

Applications: virology, biotechnology.

Collections: MWIEV