pig, kidney, subline of SPEV.
Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.
Mode of cultivation:
Conditions for cultivation:
medium - 0.5% MWPH
subculture procedure - cells detach from flask using trypsin 0.25%:
0.02% (1:9), split ratio 1:4-1:5
cryoconservation - culture medium 50%, BS 40%, DMSO 10%,
2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
78% (0 passage, dye trypan blue)
tests for bacteria, fungi were negative
2n= 38, modal number of chromosomes 40.
virus susceptibility: rota- and coronaviruses of swine