SPEV-17-91

Origin: pig, kidney, clone of SPEV.

            «Foot and Mouth Disease» 1991: 118-119. Vladimir.

Morphology: epithelial-like in monolayer

Mode of cultivation: monolayer/suspension

Conditions for cultivation: medium - BME Ca+  free

serum -   BS 10%

other components -   sodium pyruvate 1mM, glutamine 0.3 g/l

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:4

cryoconservation - culture medium 85%, BS 10%, ethylenglycol 5%,

10.0-15.0õ106 cells/ml in ampule

Viability after cryoconservation:     95-98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 38, modal number of chromosomes 40.

Other properties:

virus susceptibility: foot and mouth disease A, O, C, Asia-1.

Applications: virology, biotechnology.

Collections: MWIEV