SPEV-13-D5-TK

Origin: pig, kidney, subline of SPEV.

            Agricultur. Biology (Russ.)1985.1:20-28. USSR Patent N1275905, 1986.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:10

cryoconservation - culture medium, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     85-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 38, modal number of chromosomes 40

Other properties:

virus susceptibility: transmissible gastroenteritis of swine, equine rhinopneumonia.

Applications: virology, biotechnology.

Collections: MWIEV