SPEV

Origin: pig, embryo, kidney

            Abstr. 2nd  Sci  Conf. MNIIVP; (Russ.) 1960. 57

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMEM or DMEM or 199

serum -   FBS 10%  or BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5-1:10, optimal population density 0.9x105 cells/ml.  

cryoconservation - growth medium,10% DMSO or glycerol,

1.0-1.5106 cells/ml in ampule

Viability after cryoconservation: 90-96 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological(LDH, G6PD) analysis

Karyology:   2n= 38, variability in the range between 39-42  chromosomes,

modal number of chromosomes  40, number of markers - 1 large submetacentric

chromosome  (routine dye), number of polyploid cells 1,6%

Plating efficiency:  80%   (ESCC)

Other properties:

virus susceptibility: arbovirus A and B; entero-, rota, coronaviruses of swine,

rhinopneumonia of equine, influenza; encephalomyocarditis of swine, foot and mouth disease.

Presence of leukoviruses: Meson-Pfaizer-like and oncornaviruses.

Applications: virology, cell biology 

Collections:  MWIIW, SPBII, SPBIC, ESCC, MWIEV