SPEV-F

Origin: pig, kidney, subline of SPEV.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.3% EMPH-d

serum -    BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:4-1:5

cryoconservation - culture medium 50%, BS 40%, DMSO 10%,

3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

virus susceptibility: transmissible gastroenteritis of swine, rhinopneumonia of equine.

Applications: virology, biotechnology.

Collections: MWIEV