pig, kidney, subline of SPEV.
Mode of cultivation:
Conditions for cultivation:
medium - 0.3% EMPH-d
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split ratio 1:4-1:5
cryoconservation - culture medium 50%, BS 40%, DMSO 10%,
3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
virus susceptibility: transmissible gastroenteritis of swine, rhinopneumonia of equine.