SPEV-2

Origin: pig, embryo, kidney, clone of SPEV.

            Submitted by EVIRI, patent N 1505019, 2.02.87.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum -   BS 2%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:5, optimal population density 1.0x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.5106 cells/ml in ampule

Viability after cryoconservation:     72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 38, variability in the range between 32-64 chromosomes,

modal number of chromosomes 38.

Plating efficiency:    60%

Other properties:

virus susceptibility: arboviruses, rotavirus SA-11.

Presence of oncornaviruses A and C.

Applications: virology.

Collections: ESCC