pig, embryo, kidney, clone of SPEV.
Submitted by EVIRI, patent N 1505019, 2.02.87.
Mode of cultivation:
Conditions for cultivation:
serum - BS 2%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:5, optimal population density 1.0x105 cells/ml
cryoconservation - growth medium, 10% glycerol,
1.5õ106 cells/ml in ampule
Viability after cryoconservation:
72% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 38, variability in the range between 32-64 chromosomes,
modal number of chromosomes 38.
virus susceptibility: arboviruses, rotavirus SA-11.
Presence of oncornaviruses A and C.