Sp2/0-Ag14

Origin: mouse, myeloma, hybrid of P3X63Ag8 and mouse BALB/c spleen cells.

            Nature 1978. 276: 269; J.Immunol. 1981. 126: 317-321.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - DMEM or RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 3.0-9.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 60-66 chromosomes,

modal number of chromosomes 63-64, number of markers - 33 (differential dye).

Plating efficiency:    47% (SPBIC)

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

does not secrete Ig

Resistant to 8-azaguanine.

Applications: fusion partner for hybridomas.

Collections: ATCC CRL 1581, CRL 8287; DSM ACC 146; ECACC 86072401; SPBIC.