SK

Origin: saiga, kydney.

Abstract, the 3-d Meeting « Cultivation of Human and Animals Cells» 1990: 90.

Moscow. RF Patent N 95108959/13, 1996. Kalugina I.A., PhD thesis, Moscow, 1992.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.06% EMPH-d on Earle’s solution

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3.

cryoconservation - culture medium 50%, FBS 40%, DMSO 10%,

2.0-3.0ő106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Other properties:

chronically infected with chlamydia.

Applications: virology, biotechnology.

Collections: MWIEV.