SIRC

Origin: rabbit, cornea.

            Science 1965. 149: 633; Proc.Soc.Exp.Biol.Med. 1966. 122: 783;

Proc.Soc.Exp.Biol.Med. 1967. 125: 1271.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:3), split ratio 1:3  - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Karyology:   2n= 44, variability in the range between 51-80 chromosomes,

modal number of chromosomes 66, number of markers - 3-4 (routine dye),

 number of polyploid cells 2.5% (ATCC).

Plating efficiency:    less than 1% (ATCC)

Other properties:

virus susceptibility: rubella.

Applications: virology, cell biology.

Collections: ATCC CCL 60; ECACC 89090404; ICLC AL 96001; MWIIW; SPBIC.