SHCHS

Origin: pig, thyroid, from diploid cell line.

USSR Patent N 1347446. 1987; Annales of VIEV, 1987. 64:34-35.

Morphology:  epithelial-like  ( forms organotypical structures).

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%,

 EDTA 0.02% (1:9), split ratio 1:3-1:4.

cryoconservation - 199 50%, BS 40%, DMSO 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:   75 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 38, variability in the range between 22-60 chromosomes,

modal number of chromosomes 38.  

Other properties:

virus susceptibility: rota-, corona-, parvoviruses of swine; equine herpes 1.

Production of thyroid hormones ( thyroxin, 3-iodothyronine)

Applications:  virology , biotechnology , genetic transformation.

Collections: MWIEV.