Origin: mouse, embryo.

            Virology 1975. 65: 128.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:6, optimal population density 1.0105 cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     71-75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD)  analysis

Karyology:   2n= 40, variability in the range between73-90 chromosomes,

modal number of chromosomes 82, 20% of cells have metacentric and submetacentric

chromosomes (1-5 in a cell), number of markers - 4 acrocentric chromosomes

with additional C-bands (routine dye, C-banding).

Other properties:

virus susceptibility: N-, B- and NB-trophic murine leukemia viruses.

The cells should be used within 70-100 passages.

Applications: virology, virus titration.

Collections: ATCC CRL 1404; ECACC 86060301; ICLC ATL 96009; MWIIW.