Origin: pig, kidney.

Abstract the 2-nd Meeting «Cultivation of Human and Animals Cells» 1990: 64,

Pushchino, M.; USSR Patent N1547310, 1989.

Morphology:  epithelial-like (in monolayer)

Mode of cultivation: monolayer/stir suspension

Conditions for cultivation: medium - 0.5% EMPH-d on Earle’s solution with vitamins

for monolayer cultivation; BME - for suspension cultivation.

serum -    BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3-1:10

cryoconservation - culture medium 50%, BS 40%, DMSO 10%,

2.0-3.0õ106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Other properties:

virus susceptibility: Auesky disease; parvovirus of swine.

Applications: virology, biotechnology.

Collections: MWIEV.