Mode of cultivation:
Conditions for cultivation:
medium - EMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split ratio 1:4-1:5.
cryoconservation - EMEM 50%, FBS 40%,
DMSO 10%, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi were negative
Karyology: 2n= 44, variability in the range between 12-66 chromosomes,
modal number of chromosomes 44.
virus susceptibility: IBR, equine rhinopneumonia.