RSK

Origin: rabbit, skin.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:4-1:5.

cryoconservation - EMEM 50%, FBS 40%, DMSO 10%, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 44, variability in the range between 12-66 chromosomes,

modal number of chromosomes 44.

Other properties:

virus susceptibility: IBR, equine rhinopneumonia.

Applications: virology, biotechnology.

Collections: MWIEV