RS-88

Origin: pig, kidney.

            «Foot and Mouth Disease» Vladimir, 1991: 109. USSR Patent N 323703, 1989.

Morphology: epithelial-like in monolayer

Mode of cultivation: monolayer/suspension

Conditions for cultivation: medium - for monolayer cultivation - BME;

for cultivation in suspension - 0.25% blood protein hydrolyzate on Earle’s solution,

10x Na2HPÎ4, 2 g/l glucose; 0.3 g/l glutamine; 0.05 g/l yeast extract.

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.15%/EDTA 0.02% (1:9),

split ratio 1:6-1:8.

cryoconservation - culture medium 80%, BS 10%, DMSO 10%, 10.0-20.0õ106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 38, modal number of chromosomes 58-60.

Other properties:

virus susceptibility: foot and mouth disease, vesicular disease of swine.

Applications: virology, biotechnology.

Collections: MWIEV