«Foot and Mouth Disease» Vladimir, 1991: 109. USSR Patent N 323703, 1989.
epithelial-like in monolayer
Mode of cultivation:
Conditions for cultivation: medium - for monolayer cultivation - BME;
for cultivation in suspension - 0.25% blood protein hydrolyzate on Earle’s solution,
Na2HPÎ4, 2 g/l glucose; 0.3 g/l glutamine; 0.05 g/l yeast extract.
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.15%/EDTA 0.02% (1:9),
split ratio 1:6-1:8.
cryoconservation - culture medium 80%, BS 10%,
DMSO 10%, 10.0-20.0õ106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH and G6PD) analysis
2n= 38, modal number of chromosomes 58-60.
virus susceptibility: foot and mouth disease, vesicular disease of swine.