RLC

Origin: rat, lymphosarcoma induced by 3,3-dichlorbenzedine.

            Exp.Oncology (Russ.) 1980. 2: 40.

Morphology:  lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure  - optimal population density 5.0-7.0105 cells/ml

cryoconservation - growth medium,  10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     68% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species:  immunofluorescent and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 34-58 chromosomes,

modal number of chromosomes 38-42.

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties :

retrovirus C production

short mitotic cycle (12 human)

Applications: tumorigenicity, immunology, virology.

Collections: SPBIC.