RF Patent N 2065496, 1996.
epithelial-like and fibroblast-like
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 5-10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3) with addition of 0.5% glucose, split ratio
cryoconservation -BME 85%, BS 10%, ethylenglycol 5%, 8.0-10.0õ106 cells/ml in ampule
Viability after cryoconservation:
85-92% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH and G6PD) analysis
2n= 44, modal number of chromosomes 62
virus susceptibility: equine African plague; vesicular disease of swine.