RK-13/91

Origin: rabbit, kidney.

            RF Patent N 2065496, 1996.

Morphology: epithelial-like and fibroblast-like

Mode of cultivation: monolayer/suspension

Conditions for cultivation: medium - BME

serum -  BS 5-10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3) with addition of 0.5% glucose, split ratio 1:3.

cryoconservation -BME 85%, BS 10%, ethylenglycol 5%, 8.0-10.0106 cells/ml in ampule

Viability after cryoconservation:     85-92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 44, modal number of chromosomes 62

Other properties:

virus susceptibility: equine African plague; vesicular disease of swine.

Applications: virology, biotechnology.

Collections: MWIEV