RK13

Origin: rabbit, kidney.

            Lancet 1963. 2: 640; J. Pathol. Bacteriol. 1968. 95: 377; Annali Sclavo 1982. 24: 336.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  EMEM, BME

serum -  FBS or BS 10%

other components -  NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2 - 1:8, optimal population density 2.0-4.0x104 cells/cm2

cryoconservation - growth medium (may add 30% BS), 5-10%

DMSO, 1.0-3.0๕106 cells/ml in ampule

Viability after cryoconservation: 80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n=44, variability in the range between 62 -68  chromosomes,

modal number of chromosomes  66 , number of markers -1 large acrocentric

chromosome (routine dye), number of polyploid cells 2,6%.   

Plating efficiency:   39 % (ATCC)

Other properties:

virus susceptibility: rubella, virus B, herpes simplex, pseudorabies, vaccinia, rabbitpox,

myxoma, Simian adenovirus, vesicular stomatitis, Semliki Forest virus,

human enteroviruses, bovine rhynotracheitis.

Applications: virology.

Collections: ภาัั ััL 37; ECACC 88062427; MWIIW; SPBII; ESCC; SPBIC.