RIN m 5F

Origin: rat, insulinoma (pancreatic b-cells)

            J Biol.Chem. 1996. 271: 8307-8312.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

other components -  NEAA 1%, Hepes 1%, sodium piruvat 0,1%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3

cryoconservation - growth medium, 8-10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

insulin production

Applications:  endocrinology, cell biology.

Collections: SPBIC.