Rat 2

Origin: rat Fisher, embryo

            Virology  1981.113: 408-411.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 5-10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1-1:2), split ratio 1:3-1:5

cryoconservation - growth medium,10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Other properties:

deficient in thymidine kinase ( resistant to 5-bromodeoxyuridine)

Applications: somatic cell genetics, cell biology

Collections: ATCC CRL 1764; SPBIC.