PYAEK

Origin: cattle, tongue.

            Patent N 3845698, 17.12.84.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199 or BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:4), split ratio 1:4

cryoconservation - culture medium 70%, BS 20%, glycerol 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 60, variability in the range between 32-64 chromosomes,

modal number of chromosomes 50-54.

Other properties:

virus susceptibility: IBR, foot and mouth disease, BLV.

Applications: virology, biotechnology.

Collections: MWIEV