PTP

Origin: pig, testis.

            Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

other components - 

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 3.0-4.0106 cells/ml

Viability after cryoconservation:     74% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Other properties:

virus susceptibility: enteroviruses of swine

Applications: virology, biotechnology.

Collections: MWIEV.