PTK1 (NBL-3-17)

Origin: rat kangaroo, kidney, subline of Pt K1 (NBL-3)

            Cytology (Russ.)1988. 30: 732-738; Cytology (Russ.) 1996. 38: 75-84.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     88% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 12, variability in the range between 15-19 chromosomes,

modal number of chromosomes 17 without markers, hypotriploid, one small metacentric

of the triploid female karyotype is absent, number of polyploid cells 3%.

Applications:  cell biology, cytogenetics.

Collections: SPBIC.