rat kangaroo, kidney, subline of
Pt K1 (NBL-3)
Cytology (Russ.)1988. 30: 732-738; Cytology (Russ.) 1996. 38: 75-84.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
88% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 12, variability in the range between 15-19 chromosomes,
modal number of chromosomes 17 without markers, hypotriploid, one small metacentric
of the triploid female karyotype is absent, number of polyploid cells 3%.
cell biology, cytogenetics.